How do I submit my samples?  

Our submission process is fairly simple.  We ask that you submit your samples in an appropriate container, and ship it to us with our sample submission form (found here).  Please be sure to send the completed form as an Excel document to info@rtlgenomics.com.  If you are unclear how to fill out the form, you can contact us for more information.  Alternatively, you can submit the form with the sample names, sample labels and your contact information, and one of our specialists will contact you once the samples are received. For specific questions regarding shipping we encourage you to consult our Sample Submission Guidelines.

How long will the process take?

This question will vary depending on a number of variables.  The quality of the sample, the number of samples, if the samples need extraction, the services requested, any special instructions, and the number of projects ahead of yours.  With high quality DNA that amplifies well in the amplicon workflow the expectation is that the samples will take 3-4 weeks.  However, generally amplicon projects take 4-6 weeks.  If sample extraction is needed, the process will take an additional 1-2 weeks. Library projects vary but generally take 4-6 weeks or longer.  Check with the lab for an up to date estimate.

What is an Appropriate sample concentration, quality, and volume?

High quality samples yield higher quality results.  For submitting genomic DNA samples, we ask you determine the concentration and A260/280 ratio to know if quality is appropriate.  We request at least 20 μl volume of your sample (per assay) with a concentration around 20 ng/μl for genomic DNA or PCR amplicon.  Understanding this might present a challenge for some samples, we have success working with a wide range of concentrations and sample qualities.  Please indicate the volume and concentration of each sample you are submitting on the submission form. 

Can I do 16S sequencing on PacBio?  What is the difference between MiSeq and PacBio for 16S sequencing?

MiSeq is a short fragment sequencing process.  Because of this, you are only able to amplify a subset of the 16S (three variable regions or less) gene, but the reads are cost efficent.  The PacBio is a longer fragment platform that is capable of sequencing the full 16S gene.  This is a more expensive per read approach but provides you access to the full 16S gene.

What ARE THE STANDARD DELIVERABLES?

When your project is finished with sequencing, as well as any analyses we are doing in house, we will deliver your data via one of two mechanisms.  For relatively small datasets we will provide a secure FTP link for you to download.  For larger datasets we will send the data on an external hard drive.  We can either provide this as part of the service and you can keep the drive, or else you can send the drive back within 30 days and only pay for shipping.  Please visit our Data Analysis Methodology for further explanation about each file type.